Resumo

PECK, MT; HISS, D  e  STEPHEN, LXG. The effect of preparation method on the fibrin diameter of leukocyte- and platelet-rich fibrin (L-PRF). S. Afr. dent. j. [online]. 2018, vol.73, n.4, pp.193-197. ISSN 0375-1562.

INTRODUCTION: The use of leukocyte and platelet- rich fibrin (L-PRF) in regenerative surgery has increased exponentially in the last decade. That success has lead various centres to have introduced the biomaterial as a routine inclusion in oral grafting procedures. Evidence suggests that the physical properties of fibrin play an essential role in homeostasis of blood clots. Only a limited number of studies have investigated the features of fibrin fibre diameter of L-PRF concentrates. AIMS AND OBJECTIVES: To investigate and compare the fibrin network and fibrin fibre diameter of L-PRF prepared by two different methods. METHODS: Blood was collected from a single volunteer using established protocols. The resultant L-PRF clots were then prepared and examined using scanning electron microscopy. The results were subjected to statistical analysis. RESULTS: L-PRF prepared using the modified method had larger diameter fibrin fibres than that prepared using the official protocol. The difference was statistically significant (P=0.001). There was also a larger amount of thicker fibrin fibres observed in the modified L-PRF group. CONCLUSIONS: Preparation methods affect the fibrin fibre diameter of L-PRF. This may have consequences that influence the biological properties of the biomaterial.

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