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South African Journal of Animal Science

On-line version ISSN 2221-4062
Print version ISSN 0375-1589


VAN DER BAAN, A.; VAN NIEKERK, W.A.; RETHMAN, N.F.G.  and  COERTZE, R.J.. The determination of digestibility of Atriplex nummularia cv. De Kock (Oldman's Saltbush) using different in vitro techniques. S. Afr. j. anim. sci. [online]. 2004, vol.34, n.5, pp.95-97. ISSN 2221-4062.

The main disadvantages of the rumen fluid in vitro technique are the cost and welfare issues of keeping cannulated animals. The purpose of the study was to find an accurate alternative in vitro technique to determine organic matter digestibility (OMD) of Atriplex nummularia supplemented with two energy sources. The in vitro faeces technique of El Shaer et al. (1987) is an easier and cheaper alternative to the classic rumen fluid in vitro technique of Tilley & Terry (1963), as modified by Engels & Van der Merwe (1967). The in vitro gas production technique of Piennaar (1994), the cellulase in vitro techniques of De Boever et al. (1986) and the modified Wageningen one, were not as accurate determining OMD as the in vivo technique.

Keywords : In vitro techniques; rumen and faeces inoculum; gas production; cellulase; Atriplex.

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